Ultra-low frequency labeling of single cell lineages in Drosophila.
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ABSTRACT:
SUBMITTER: Snee M
PROVIDER: S-EPMC7205028 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Publications
Ultra-low frequency labeling of single cell lineages in Drosophila.
Snee Mark M Lee Wen-Chih WC Beehler-Evans Ryan R Micchelli Craig A CA
Developmental biology 20190921 1
We describe a new methodology for genetically labeling single cell lineages in Drosophila called DMARCM. The system offers ultra-low frequency labeling, linear induction, consistent labeling among individuals and virtually no background signal. We compare this technique to an existing approach, which has been widely adopted. We demonstrate how application of DMARCM in the gastrointestinal epithelium permits the effects of labeling frequency on tumorigenic stem cell growth to be distinguished in ...[more]
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