Ontology highlight
ABSTRACT:
SUBMITTER: O'Huallachain M
PROVIDER: S-EPMC7205613 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
O'Huallachain Maeve M Bava Felice-Alessio FA Shen Mary M Dallett Carolina C Paladugu Sri S Samusik Nikolay N Yu Simon S Hussein Razika R Hillman Grantland R GR Higgins Samuel S Lou Melanie M Trejo Angelica A Qin Laura L Tai Yu Chuan YC Kinoshita Shigemi M SM Jager Astraea A Lashkari Deval D Goltsev Yury Y Ozturk Sedide S Nolan Garry P GP
Communications biology 20200507 1
Single-cell omics provide insight into cellular heterogeneity and function. Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (f ...[more]