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Optimization studies on biodegradation of atrazine by Bacillus badius ABP6 strain using response surface methodology.

ABSTRACT: In this study, the optimization of distinctive environmental factors such as pH, temperature, agitation-speed and atrazine-concentration on atrazine degradation by utilizing Bacillus badius ABP6 strain, has been done through response-surface-methodology (RSM). The optimum-conditions after analysis for the maximum atrazine degradation were: pH 7.05, temperature 30.4?°C, agitation-speed 145.7?rpm, and atrazine-concentration 200.9?ppm. The prescribed model was approved for high F-value (95.92), very low P-value (<0.01) and non- significant lack of fit (0.1627). It was observed that under the optimized-conditions, the R2 value of regression models for all the response variables was 0.9897 and the maximum atrazine degradation i.e. 89.7 % was found. Finally for graphical representation, the validated optimum-conditions of variables and responses were simulated using three dimensional plots (3D). The confirmation of the model is successful to suggest the optimization parameters of atrazine degradation under in-situ condition by bacterial isolate employing response-surface-methodology optimization tool of Design expert software (new version 10.0.1).

SUBMITTER: Khatoon H

PROVIDER: S-EPMC7210405 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Optimization studies on biodegradation of atrazine by <i>Bacillus badius</i> ABP6 strain using response surface methodology.

Khatoon Hina H Rai J P N JPN

Biotechnology reports (Amsterdam, Netherlands) 20200428

In this study, the optimization of distinctive environmental factors such as pH, temperature, agitation-speed and atrazine-concentration on atrazine degradation by utilizing <i>Bacillus badius</i> ABP6 strain, has been done through response-surface-methodology (RSM). The optimum-conditions after analysis for the maximum atrazine degradation were: pH 7.05, temperature 30.4 °C, agitation-speed 145.7 rpm, and atrazine-concentration 200.9 ppm. The prescribed model was approved for high F-value (95.9 ...[more]

PMID: 32395437

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Project description:BackgroundPolyhydroxybutyrate (PHB) is a biopolymer formed by some microbes in response to excess carbon sources or essential nutrient depletion. PHBs are entirely biodegradable into CO2 and H2O under aerobic and anaerobic conditions. It has several applications in various fields such as medicine, pharmacy, agriculture, and food packaging due to its biocompatibility and nontoxicity nature.ResultIn the present study, PHB-producing bacterium was isolated from the Dirout channel at Assiut Governorate. This isolate was characterized phenotypically and genetically as Bacillus cereus SH-02 (OM992297). According to one-way ANOVA test, the maximum PHB content was observed after 72 h of incubation at 35 °C using glucose and peptone as carbon and nitrogen source. Response surface methodology (RSM) was used to study the interactive effects of glucose concentration, peptone concentration, and pH on PHB production. This result proved that all variables have a significant effect on PHB production either independently or in the interaction with each other. The optimized medium conditions with the constraint to maximize PHB content and concentration were 22.315 g/L glucose, and 15.625 g/L peptone at pH 7.048. The maximum PHB content and concentration were 3100.799 mg/L and 28.799% which was close to the actual value (3051 mg/l and 28.7%). The polymer was identified as PHB using FTIR, NMR, and mass spectrometry. FT-IR analysis showed a strong band at 1724 cm- 1 which attributed to the ester group's carbonyl while NMR analysis has different peaks at 169.15, 67.6, 40.77, and 19.75 ppm that were corresponding to carbonyl, methine, methylene, and methyl resonance. Mass spectroscopy exhibited molecular weight for methyl 3- hydroxybutyric acid.ConclusionPHB-producing strain was identified as Bacillus cereus SH-02 (OM992297). Under optimum conditions from RSM analysis, the maximum PHB content and concentration of this strain can reach (3100.799 mg/L and 28.799%); respectively. FTIR, NMR, and Mass spectrometry were used to confirm the polymer as PHB. Our results demonstrated that optimization using RSM is one of the strategies used for reducing the production cost. RSM can determine the optimal factors to produce the polymer in a better way and in a larger quantity without consuming time.

Dataset Information

Optimization studies on biodegradation of atrazine by Bacillus badius ABP6 strain using response surface methodology.

Publications

Optimization studies on biodegradation of atrazine by <i>Bacillus badius</i> ABP6 strain using response surface methodology.

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