Ontology highlight
ABSTRACT:
SUBMITTER: Vinogradov AA
PROVIDER: S-EPMC7210931 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Vinogradov Alexander A AA Shimomura Morito M Goto Yuki Y Ozaki Taro T Asamizu Shumpei S Sugai Yoshinori Y Suga Hiroaki H Onaka Hiroyasu H
Nature communications 20200508 1
Lactazole A is a cryptic thiopeptide from Streptomyces lactacystinaeus, encoded by a compact 9.8 kb biosynthetic gene cluster. Here, we establish a platform for in vitro biosynthesis of lactazole A, referred to as the FIT-Laz system, via a combination of the flexible in vitro translation (FIT) system with recombinantly produced lactazole biosynthetic enzymes. Systematic dissection of lactazole biosynthesis reveals remarkable substrate tolerance of the biosynthetic enzymes and leads to the develo ...[more]