Ontology highlight
ABSTRACT:
SUBMITTER: Ravindra Kumar S
PROVIDER: S-EPMC7219404 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Ravindra Kumar Sripriya S Miles Timothy F TF Chen Xinhong X Brown David D Dobreva Tatyana T Huang Qin Q Ding Xiaozhe X Luo Yicheng Y Einarsson Pétur H PH Greenbaum Alon A Jang Min J MJ Deverman Benjamin E BE Gradinaru Viviana V
Nature methods 20200420 5
Recombinant adeno-associated viruses (rAAVs) are efficient gene delivery vectors via intravenous delivery; however, natural serotypes display a finite set of tropisms. To expand their utility, we evolved AAV capsids to efficiently transduce specific cell types in adult mouse brains. Building upon our Cre-recombination-based AAV targeted evolution (CREATE) platform, we developed Multiplexed-CREATE (M-CREATE) to identify variants of interest in a given selection landscape through multiple positive ...[more]