Bok regulates mitochondrial fusion and morphology.

ABSTRACT: Bok (Bcl-2-related ovarian killer) is a member of the Bcl-2 protein family that governs the intrinsic apoptosis pathway, but the cellular role that Bok plays is controversial. Remarkably, endogenous Bok is constitutively bound to inositol 1,4,5-trisphosphate receptors (IP₃Rs) and is stabilized by this interaction. Here we report that despite the strong association with IP₃Rs, deletion of Bok expression by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease)-mediated gene editing does not alter calcium mobilization via IP₃Rs or calcium influx into the mitochondria. Rather, Bok deletion significantly reduces mitochondrial fusion rate, resulting in mitochondrial fragmentation. This phenotype is reversed by exogenous wild-type Bok and by an IP₃R binding-deficient Bok mutant, and may result from a decrease in mitochondrial motility. Bok deletion also enhances mitochondrial spare respiratory capacity and membrane potential. Finally, Bok does not play a major role in apoptotic signaling, since Bok deletion does not alter responsiveness to various apoptotic stimuli. Overall, despite binding to IP₃Rs, Bok does not alter IP₃R-mediated Ca²⁺ signaling, but is required to maintain normal mitochondrial fusion, morphology, and bioenergetics.

SUBMITTER: Schulman JJ 

PROVIDER: S-EPMC7224202 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature