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Creating New ?-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences.


ABSTRACT: Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of cis-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral vectors has proven challenging. In this study, we deploy LV-MPRA (lentiviral vector-based, massively parallel reporter assay), an approach that simultaneously analyzes thousands of synthetic DNA fragments in parallel to identify sequence-intrinsic and lineage-specific enhancer function at near-base-pair resolution. We demonstrate the power of LV-MPRA in elucidating the boundaries of previously unknown intrinsic enhancer sequences of the human ?-globin locus control region. Our approach facilitated the rapid assembly of novel therapeutic ?AS3-globin lentiviral vectors harboring strong lineage-specific recombinant control elements capable of correcting a mouse model of sickle cell disease. LV-MPRA can be used to map any genomic locus for enhancer activity and facilitates the rapid development of therapeutic vectors for treating disorders of the hematopoietic system or other specific tissues and cell types.

SUBMITTER: Morgan RA 

PROVIDER: S-EPMC7225380 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences.

Morgan Richard A RA   Ma Feiyang F   Unti Mildred J MJ   Brown Devin D   Ayoub Paul George PG   Tam Curtis C   Lathrop Lindsay L   Aleshe Bamidele B   Kurita Ryo R   Nakamura Yukio Y   Senadheera Shantha S   Wong Ryan L RL   Hollis Roger P RP   Pellegrini Matteo M   Kohn Donald B DB  

Molecular therapy. Methods & clinical development 20200418


Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of <i>cis</i>-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral vectors has proven challenging. In this study, we deploy LV-MPRA (lentiviral vector-based, massively parallel reporter assay), an approach that simultaneously analyzes thousands of synthetic DNA fragments in parallel to identify sequence-intrin  ...[more]

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