Ontology highlight
ABSTRACT:
SUBMITTER: Du Y
PROVIDER: S-EPMC7229031 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Du Yushen Y Hultquist Judd F JF Zhou Quan Q Olson Anders A Tseng Yenwen Y Zhang Tian-Hao TH Hong Mengying M Tang Kejun K Chen Liubo L Meng Xiangzhi X McGregor Michael J MJ Dai Lei L Gong Danyang D Martin-Sancho Laura L Chanda Sumit S Li Xinming X Bensenger Steve S Krogan Nevan J NJ Sun Ren R
Nature communications 20200515 1
A comprehensive examination of protein-protein interactions (PPIs) is fundamental for the understanding of cellular machineries. However, limitations in current methodologies often prevent the detection of PPIs with low abundance proteins. To overcome this challenge, we develop a mRNA display with library of even-distribution (md-LED) method that facilitates the detection of low abundance binders with high specificity and sensitivity. As a proof-of-principle, we apply md-LED to IAV NS1 protein. ...[more]