Ontology highlight
ABSTRACT:
SUBMITTER: Smith CJ
PROVIDER: S-EPMC7229841 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Smith Cory J CJ Castanon Oscar O Said Khaled K Volf Verena V Khoshakhlagh Parastoo P Hornick Amanda A Ferreira Raphael R Wu Chun-Ting CT Güell Marc M Garg Shilpa S Ng Alex H M AHM Myllykallio Hannu H Church George M GM
Nucleic acids research 20200501 9
To extend the frontier of genome editing and enable editing of repetitive elements of mammalian genomes, we made use of a set of dead-Cas9 base editor (dBE) variants that allow editing at tens of thousands of loci per cell by overcoming the cell death associated with DNA double-strand breaks and single-strand breaks. We used a set of gRNAs targeting repetitive elements-ranging in target copy number from about 32 to 161 000 per cell. dBEs enabled survival after large-scale base editing, allowing ...[more]