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Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin.


ABSTRACT: BACKGROUND:Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. METHODS:Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and ?-carotene-linoleate bleaching assay. The ?-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while ?-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-?-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. RESULTS:Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good ?-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of ?-glucosidase activity (82.11. ± 2.15%). CONCLUSION:These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.

SUBMITTER: Chokki M 

PROVIDER: S-EPMC7230926 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of <i>Morinda lucida</i> and <i>Momordica charantia</i> Leaves from Benin.

Chokki Michaelle M   Cudălbeanu Mihaela M   Zongo Cheikna C   Dah-Nouvlessounon Durand D   Ghinea Ioana Otilia IO   Furdui Bianca B   Raclea Robert R   Savadogo Aly A   Baba-Moussa Lamine L   Avamescu Sorin Marius SM   Dinica Rodica Mihaela RM   Baba-Moussa Farid F  

Foods (Basel, Switzerland) 20200404 4


<h4>Background</h4><i>Momordica charantia</i> Linn. (<i>Cucurbitaceae</i>), the wild variety of bitter melon and <i>Morinda lucida</i> Benth (<i>Rubiaceae</i>) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of <i>M. charantia</i> and <i>M. lucida</i> leaves and their antidiabetic activity.<h4>Methods</h4>Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activ  ...[more]

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