Ontology highlight
ABSTRACT:
SUBMITTER: Woelfl F
PROVIDER: S-EPMC7232479 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Woelfl Franziska F Léger Psylvia P Oreshkova Nadia N Pahmeier Felix F Windhaber Stefan S Koch Jana J Stanifer Megan M Roman Sosa Gleyder G Uckeley Zina M ZM Rey Felix A FA Boulant Steeve S Kortekaas Jeroen J Wichgers Schreur Paul J PJ Lozach Pierre-Yves PY
Viruses 20200404 4
The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant ...[more]