Ontology highlight
ABSTRACT:
SUBMITTER: Kuksa PP
PROVIDER: S-EPMC7233376 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Kuksa Pavel P PP Leung Yuk Yee YY Vandivier Lee E LE Anderson Zachary Z Gregory Brian D BD Wang Li-San LS
Methods in molecular biology (Clifton, N.J.) 20170101
RNA molecules are often altered post-transcriptionally by the covalent modification of their nucleotides. These modifications are known to modulate the structure, function, and activity of RNAs. When reverse transcribed into cDNA during RNA sequencing library preparation, atypical (modified) ribonucleotides that affect Watson-Crick base pairing will interfere with reverse transcriptase (RT), resulting in cDNA products with mis-incorporated bases or prematurely terminated RNA products. These inte ...[more]