Ontology highlight
ABSTRACT:
SUBMITTER: Perez-Alvarez A
PROVIDER: S-EPMC7235013 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Perez-Alvarez Alberto A Fearey Brenna C BC O'Toole Ryan J RJ Yang Wei W Arganda-Carreras Ignacio I Lamothe-Molina Paul J PJ Moeyaert Benjamien B Mohr Manuel A MA Panzera Lauren C LC Schulze Christian C Schreiter Eric R ER Wiegert J Simon JS Gee Christine E CE Hoppa Michael B MB Oertner Thomas G TG
Nature communications 20200518 1
Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presyna ...[more]