Cells grown in three-dimensional spheroids mirror in vivo metabolic response of epithelial cells.

ABSTRACT: Metabolism in cells adapts quickly to changes in nutrient availability and cellular differentiation status, including growth conditions in cell culture settings. The last decade saw a vast increase in three-dimensional (3D) cell culture techniques, engendering spheroids and organoids. These methods were established to improve comparability to in vivo situations, differentiation processes and growth modalities. How far spheroids mimic in vivo metabolism, however, remains enigmatic. Here, to our knowledge, we compare for the first time metabolic fingerprints between cells grown as a single layer or as spheroids with freshly isolated in situ tissue. While conventionally grown cells express elevated levels of glycolysis intermediates, amino acids and lipids, these levels were significantly low

SUBMITTER: Lagies S 

PROVIDER: S-EPMC7237469 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature