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Decolorization of Palm Oil Mill Effluent by Klebsiella Pneumonia ABZ11: Remediation Efficacy and Statistical Optimization of Treatment Conditions.

ABSTRACT: Colorants contained in palm oil mill effluent (POME) are recalcitrant and carcinogenic in nature. The commonly applied ponding treatment methods have been reported inefficient for remediating the concentration of the colorants before discharge. The need for sustainable and efficient treatment technique is crucial in order to preserve the environment. In this view, this study reported the first attempt to decolorize POME using a proliferate Klebsiella Pneumonia ABZ11 at varied inoculum sizes of 5-25% (v/v), initial color concentration (650-2,600 ADMI) and treatment time of 5-40 h. The treatment conditions were optimized using Response Surface Methodology. At optimal conditions of 20% (v/v) inoculum size, initial-color concentration of 2,600 ADMI, initial pH of 7 and 35 h treatment retention time, over 80.40% color removal was achieved with insignificant disparity compared with the model predicted value of 81.538%. Also, the Monod model excellently described the decolorization kinetic process with 0.9214 coefficient of correlation (R 2), and the calculated maximum growth ? max ) and half-saturation constant (K s ) were 7.023 d-1 and 340.569 ADMI d-1, respectively. This study revealed that the Klebsiella Pneumonia ABZ11 was highly prolific and such feature may favor a synergistic biodegradation process.

SUBMITTER: Abdulsalam M

PROVIDER: S-EPMC7237724 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Decolorization of Palm Oil Mill Effluent by <i>Klebsiella Pneumonia</i> ABZ11: Remediation Efficacy and Statistical Optimization of Treatment Conditions.

Abdulsalam Mohammed M Man Hasfalina Che HC Abidin Zurina Zainal ZZ Yunos Khairul Faezah KF Idris Aida Isma AI

Frontiers in microbiology 20200513

Colorants contained in palm oil mill effluent (POME) are recalcitrant and carcinogenic in nature. The commonly applied ponding treatment methods have been reported inefficient for remediating the concentration of the colorants before discharge. The need for sustainable and efficient treatment technique is crucial in order to preserve the environment. In this view, this study reported the first attempt to decolorize POME using a proliferate <i>Klebsiella Pneumonia</i> ABZ11 at varied inoculum siz ...[more]

PMID: 32477278

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Project description:BackgroundThermophilic Bacillus strains of phylogenetic Bacillus rRNA group 5 were described as a new genus Geobacillus. Their geographical distribution included oilfields, hay compost, hydrothermal vent or soils. The members from the genus Geobacillus have a growth temperatures ranging from 35 to 78 degrees C and contained iso-branched saturated fatty acids (iso-15:0, iso-16:0 and iso-17:0) as the major fatty acids. The members of Geobacillus have similarity in their 16S rRNA gene sequences (96.5-99.2%). Thermophiles harboring intrinsically stable enzymes are suitable for industrial applications. The quest for intrinsically thermostable lipases from thermophiles is a prominent task due to the laborious processes via genetic modification.ResultsTwenty-nine putative lipase producers were screened and isolated from palm oil mill effluent in Malaysia. Of these, isolate T1T was chosen for further study as relatively higher lipase activity was detected quantitatively. The crude T1 lipase showed high optimum temperature of 70 degrees C and was also stable up to 60 degrees C without significant loss of crude enzyme activity. Strain T1T was a Gram-positive, rod-shaped, endospore forming bacterium. On the basic of 16S rDNA analysis, strain T1T was shown to belong to the Bacillus rRNA group 5 related to Geobacillus thermoleovorans (DSM 5366T) and Geobacillus kaustophilus (DSM 7263T). Chemotaxonomic data of cellular fatty acids supported the affiliation of strain T1T to the genus Geobacillus. The results of physiological and biochemical tests, DNA/DNA hybridization, RiboPrint analysis, the length of lipase gene and protein pattern allowed genotypic and phenotypic differentiation of strain T1T from its validly published closest phylogenetic neighbors. Strain T1T therefore represents a novel species, for which the name Geobacillus zalihae sp. nov. is proposed, with the type strain T1T (=DSM 18318T; NBRC 101842T).ConclusionStrain T1T was able to secrete extracellular thermostable lipase into culture medium. The strain T1T was identified as Geobacillus zalihae T1T as it differs from its type strains Geobacillus kaustophilus (DSM 7263T) and Geobacillus thermoleovorans (DSM 5366T) on some physiological studies, cellular fatty acids composition, RiboPrint analysis, length of lipase gene and protein profile.

Dataset Information

Decolorization of Palm Oil Mill Effluent by Klebsiella Pneumonia ABZ11: Remediation Efficacy and Statistical Optimization of Treatment Conditions.

Publications

Decolorization of Palm Oil Mill Effluent by <i>Klebsiella Pneumonia</i> ABZ11: Remediation Efficacy and Statistical Optimization of Treatment Conditions.

Similar Datasets

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