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Self-Assembly Behaviors of a Penta-Phenylene Maltoside and Its Application for Membrane Protein Study.

ABSTRACT: We prepared an amphiphile with a penta-phenylene lipophilic group and a branched trimaltoside head group. This new agent, designated penta-phenylene maltoside (PPM), showed a marked tendency to self-assembly into micelles via strong aromatic-aromatic interactions in aqueous media, as evidenced by 1 H?NMR spectroscopy and fluorescence studies. When utilized for membrane protein studies, this new agent was superior to DDM, a gold standard conventional detergent, in stabilizing multiple proteins long term. The ability of this agent to form aromatic-aromatic interactions is likely responsible for enhanced protein stabilization when associated with a target membrane protein.

SUBMITTER: Ehsan M

PROVIDER: S-EPMC7239035 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Self-Assembly Behaviors of a Penta-Phenylene Maltoside and Its Application for Membrane Protein Study.

Ehsan Muhammad M Kumar Ashwani A Mortensen Jonas S JS Du Yang Y Hariharan Parameswaran P Kumar Kaavya K KK Ha Betty B Byrne Bernadette B Guan Lan L Kobilka Brian K BK Loland Claus J CJ Chae Pil Seok PS

Chemistry, an Asian journal 20190410 11

We prepared an amphiphile with a penta-phenylene lipophilic group and a branched trimaltoside head group. This new agent, designated penta-phenylene maltoside (PPM), showed a marked tendency to self-assembly into micelles via strong aromatic-aromatic interactions in aqueous media, as evidenced by <sup>1</sup> H NMR spectroscopy and fluorescence studies. When utilized for membrane protein studies, this new agent was superior to DDM, a gold standard conventional detergent, in stabilizing multiple ...[more]

PMID: 30969484

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