Ontology highlight
ABSTRACT:
SUBMITTER: Choe H
PROVIDER: S-EPMC7239952 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Choe Hyunjun H Cha Minsun M Stewart Jon D JD
Enzyme and microbial technology 20200121
Serine palmitoyltransferase (SPTase), the first enzyme of the sphingolipid biosynthesis pathway, produces 3-ketodihydrosphingosine by a Claisen-like condensation/decarboxylation reaction of l-Ser and palmitoyl-CoA (n-C<sub>16</sub>-CoA). Previous structural analysis of Sphingomonas paucimobilis SPTase (SpSPTase) revealed a dynamic active site loop (RPPATP; amino acids 378-383) in which R378 (underlined) forms a salt bridge with the carboxylic acid group of the PLP : l-Ser external aldimine. We h ...[more]