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Digital multiplex ligation assay for highly multiplexed screening of ?-lactamase-encoding genes in bacterial isolates.

ABSTRACT: Increasing incidence of antibiotic resistance in clinical and environmental settings calls for increased scalability in their surveillance. Current screening technologies are limited by the number of samples and genes that can easily be screened. We demonstrate here digital multiplex ligation assay (dMLA) as a low-cost targeted genomic detection workflow capable of highly-parallel screening of bacterial isolates for multiple target gene regions simultaneously. Here, dMLA is used for simultaneous detection of 1187 ?-lactamase-encoding genes, including extended spectrum ?-lactamase (ESBL) genes, in 74 bacterial isolates. We demonstrate dMLA as a light-weight and cost-efficient workflow which provides a highly scalable tool for antimicrobial resistance surveillance and is also adaptable to genetic screening applications beyond antibiotic resistance.

SUBMITTER: Tamminen M

PROVIDER: S-EPMC7248093 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Digital multiplex ligation assay for highly multiplexed screening of β-lactamase-encoding genes in bacterial isolates.

Tamminen Manu M Spaak Jenny J Caduff Lea L Schiff Hanna H Lang Ramon R Schmid Steven S Montealegre Maria Camila MC Julian Timothy R TR

Communications biology 20200525 1

Increasing incidence of antibiotic resistance in clinical and environmental settings calls for increased scalability in their surveillance. Current screening technologies are limited by the number of samples and genes that can easily be screened. We demonstrate here digital multiplex ligation assay (dMLA) as a low-cost targeted genomic detection workflow capable of highly-parallel screening of bacterial isolates for multiple target gene regions simultaneously. Here, dMLA is used for simultaneous ...[more]

PMID: 32451431

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