Browse
Submit Data
Databases
API
Help

Dataset Information

7 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A new species of buffalo leech in the genus Hirudinaria Whitman, 1886 (Arhynchobdellida, Hirudinidae) from Thailand.

ABSTRACT: Hirudinaria manillensis (Lesson, 1842), commonly known as the buffalo leech, shows a polymorphism of two ventral colorations. The green color morph has a plain green ventral surface and the red color morph has a brick-red ventral surface with two black submarginal stripes. Based on molecular and morphological evidence in the present study, these two color morphs were revealed as two different species. The red color morph fits well with the description of H. manillensis, while the green color morph showed some distinctions, and therefore is described herein as Hirudinaria thailandica Jeratthitikul & Panha, sp. nov. The new species can be distinguished from its congeners by the dark greenish or dark olive ventral surface and a round atrium with ventral insertion of ejaculatory ducts in the male reproductive organ. A phylogenetic tree based on concatenated data of COI and 28S genes supported the new species and further indicated it as a sister species to H. bpling Phillips, 2012.

SUBMITTER: Jeratthitikul E

PROVIDER: S-EPMC7248127 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Systematics of the freshwater leech genus Hirudinaria Whitman, 1886 (Arhynchobdellida, Hirudinidae) from northeastern Thailand.

Project description:In total, 435 specimens of the Southeast Asian freshwater leech species within the Hirudinidae family were collected from 17 locations of various types of aquatic habitats in northeastern Thailand. They were all morphologically placed within the genus Hirudinaria Whitman, 1886 and there were three distinct species: the common Hirudinariamanillensis, 78.2% of all collected specimens and at all 17 locations, Hirudinariajavanica at 20.3% of collected samples and from five locations and a rarer unidentified morphospecies (Hirudinaria sp.) with six samples from only two locations. The karyotypes of these three species were examined across their range in this study area for 38, 11 and 6 adult specimens of Hirudinariamanillensis, Hirudinariajavanica and Hirudinaria sp., respectively. This revealed different chromosome numbers among all three species, with Hirudinariajavanica having n = 13, 2n = 26, Hirudinariamanillensis lacked one small chromosome pair with n = 12, 2n = 24, and the unknown Hirudinaria sp. differed from any known Hirudinaria karyotypes in exhibiting a higher chromosome number (n = 14, 2n = 28) and a gradual change in size from large to small chromosomes. This suggests that the unknown Hirudinaria sp. is a new biological species. However, phylogenetic analysis based upon a 658 bp fragment of the cytochrome oxidase subunit I gene placed this unknown morphospecies within the Hirudinariamanillensis clade, perhaps then suggesting a recent sympatric speciation, although this requires further confirmation. Regardless, the chromosomes of all three species were asymmetric, most with telocentric elements. A distinct bi-armed chromosome marker was present on the first chromosome pair in Hirudinariajavanica, whilst it was on pairs 1, 2, 3 and 5 in Hirudinariamanillensis, and on pairs 3 and 5 for the unknown Hirudinaria sp.

| S-EPMC4258631 | biostudies-literature

Biodiversity of the Buffalo Leeches Genus Hirudinaria (Arhynchobdellida, Hirudinidae) in Southern Thailand Revealed from DNA Barcoding.

Project description:Leeches in the genus Hirudinaria Whitman, 1886, also known as buffalo leeches, are blood-sucking ectoparasites of vertebrates. Although they are widely distributed in Asia and had been highly abundant in the past, studies on diversity and taxonomy of this genus are still scarce. There is probably a large amount of cryptic diversity yet to be discovered, particularly from mainland Southeast Asia. In this study, we used morphology and DNA barcoding with a COI gene fragment to explored the diversity of Hirudinaria leeches in the southern region of Thailand, where a unique geographic feature could have led to the diversification of freshwater biota. Molecular phylogenetic analyses and species delimitation approaches (ABGD, bPTP, GMYC, and BOLD) revealed the presence of four putative species of Hirudinaria leeches from southern Thailand, including H. bpling, H. thailandica, and two morphologically cryptic lineages of H. manillensis. Compared to other leech genera, genetic distances of Hirudinaria leeches were relatively low (0.11-0.65% within species; 3.72-14.36% between species) and barcoding gaps were very narrow (1.54-2.88%). The species diversity, distribution pattern, and a phenomenon of low genetic divergence of Hirudinaria leeches in southern Thailand could be explained by an ancient seaway, paleo-drainage, and anthropogenic activities.

| S-EPMC10064040 | biostudies-literature

Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis.

Project description:The Asian Buffalo leech, Hirudinaria manillensis, is an aquatic sanguivorous species distributed widely in Southeast Asia. H. manillensis has long been used clinically for bloodletting and other medical purposes. Recent studies have focused on artificial culturing, strain optimization, and the identification and development new drugs based on the anticoagulant effects of H. manillensis bites; however, data regarding its genome remain unclear. This study aimed to determine the genome sequence of an adult Asian Buffalo leech. We generated a draft assembly of 151.8 Mb and a N50 scaffold of 2.28 Mb. Predictions indicated that the assembled genome contained 21,005 protein-coding genes. Up to 17,865 genes were annotated in multiple databases including Gene Ontology. Sixteen anticoagulant proteins with a Hirudin or Antistasin domain were identified. This study is the first to report the whole-genome sequence of the Asian Buffalo leech, an important sanguivorous leech of clinical significance. The quality of the assembly is comparable to those of other annelids. These data will help further the current understanding of the biological mechanisms and genetic characteristics of leeches and serve as a valuable resource for future studies.

| S-EPMC6977106 | biostudies-literature

Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis.

Project description:Protease inhibitors have been reported rarely from the leech Hirudinaria manillensis. In this study, we purified a novel protease inhibitor (bdellin-HM-2) with anticoagulant properties from H. manillensis. With a molecular weight of 1.4x104, bdellin-HM-2 was also characterized with three intra-molecular disulfide bridges at the N-terminus and multiple HHXDD and HXDD motifs at the C-terminus. cDNA cloning revealed that the putative nucleotide-encoding protein of bdellin-HM-2 contained 132 amino acids and was encoded by a 399 bp open reading frame (ORF). Sequence alignment showed that bdellin-HM-2 shared similarity with the "non-classical" Kazal-type serine protease inhibitors, but had no inhibitory effect on trypsin, elastase, chymotrypsin, kallikrein, factor XIIa (FXIIa), factor XIa (FXIa), factor Xa (FXa), thrombin, or plasmin. Bdellin-HM-2 showed anticoagulant effects by prolonging the activated partial thromboplastin time (aPTT), indicating a role in enabling H. manillensis to obtain a blood meal from its host. Our results suggest that bdellin-HM-2 may play a crucial role in blood-sucking in this leech species and may be a potential candidate for the development of clinical anti-thrombotic drugs.

| S-EPMC6591161 | biostudies-literature

Purification and Characterization of a Novel Kazal-Type Trypsin Inhibitor from the Leech of Hirudinaria manillensis.

Project description:Kazal-type serine proteinase inhibitors are found in a large number of living organisms and play crucial roles in various biological and physiological processes. Although some Kazal-type serine protease inhibitors have been identified in leeches, none has been reported from Hirudinaria manillensis, which is a medically important leech. In this study, a novel Kazal-type trypsin inhibitor was isolated from leech H. manillensis, purified and named as bdellin-HM based on the sequence similarity with bdellin-KL and bdellin B-3. Structural analysis revealed that bdellin-HM was a 17,432.8 Da protein and comprised of 149 amino acid residues with six cysteines forming three intra-molecular disulfide bonds. Bdellin-HM showed similarity with the Kazal-type domain and may belong to the group of "non-classical" Kazal inhibitors according to its Cys(I)-Cys(II) disulfide bridge position. Bdellin-HM had no inhibitory effect on elastase, chymotrypsin, kallikrein, Factor (F) XIIa, FXIa, FXa, thrombin and plasmin, but it showed a potent ability to inhibit trypsin with an inhibition constant (Ki) of (8.12 ± 0.18) × 10(-9) M. These results suggest that bdellin-HM from the leech of H. manillensis plays a potent and specific inhibitory role towards trypsin.

| S-EPMC4999845 | biostudies-literature

Characterization of the complete mitogenome of a land leech, Haemadipsa crenata Ngamprasertwong (Arhynchobdellida: Haemadipsidae).

Project description:Land leeches of genus Haemadipsa (Family Haemadipsidae) are widely distributed in South East Asia. Haemadipsa crenata Ngamprasertwong is a blood-feeding species firstly reported from Thailand. A complete mitochondrial genome of H. crenata was characterized in this study for further genetic exploration on land leech. The reads were assembled into a circular mitogenome of 14,725 bp in length. The AT content of H. crenata mitogenome is 76.79%. The annotated mitogenome contains 22 tRNAs, 2 rRNAs, and 13 protein-coding genes (PCGs), and the structure of PCG open reading frames was confirmed. Finally, the phylogenetic relationship of H. crenata and other leech species were reconstructed using mitogenomes.

| S-EPMC8218832 | biostudies-literature

Salticidae (Arachnida, Araneae) of Thailand: new species and records of Epeus Peckham & Peckham, 1886 and Ptocasius Simon, 1885.

Project description:The study is based on new material from the collections of the Naturalis Biodiversity Centre in Leiden (RNHM) and the Hungarian Natural History Museum (HNHM) and addresses issues in two genera: Epeus Peckham & Peckham, 1886 and Ptocasius Simon, 1885 from Thailand. Both genera are of Asian/Indomalayan origin, the latter with a diversity hotspot in the subtropical valleys of the Himalayas. Based on morphological data, we propose three new species of Epeus (Epeus daiqini sp. nov. (??), Epeus pallidus sp. nov. (?), Epeus szirakii sp. nov. (?)) and two new species of Ptacasius (Ptocasius metzneri sp. nov. (??) and Ptocasius sakaerat sp. nov. (?)). Additionally, we redescribed E. tener (Simon, 1877) and added photographs of morphological characters. The genus Ptocasius is redefined due to the inclusion of 37 species, previously included in Yaginumaella Prószy?ski, 1979. Relationships and distribution of both genera are discussed in reference to molecular, morphological and distributional data, published by other authors in recent years.

| S-EPMC7316081 | biostudies-literature

Short tail stories: the hirudin-like factors HLF6 and HLF7 of the Asian medicinal leech, Hirudinaria manillensis.

Project description:The leech-derived hirudins and hirudin-like factors (HLFs) share a common molecule structure: a short N-terminus, a central globular domain, and an elongated C-terminal tail. All parts are important for function. HLF6 and HLF7 were identified in the Asian medicinal leech, Hirudinaria manillensis. The genes of both factors encode putative splice variants that differ in length and composition of their respective C-terminal tails. In either case, the tails are considerably shorter compared to hirudins. Here we describe the functional analyses of the natural splice variants and of synthetic variants that comprise an altered N-terminus and/or a modified central globular domain. All natural splice variants of HLF6 and HLF7 display no detectable thrombin-inhibitory potency. In contrast, some synthetic variants effectively inhibit thrombin, even with tails as short as six amino acid residues in length. Our data indicate that size and composition of the C-terminal tail of hirudins and HLFs can vary in a great extent, yet the full protein may still retain the ability to inhibit thrombin.

| S-EPMC8516769 | biostudies-literature

Six new species of the genus Exocelina Broun, 1886 from Wano Land, New Guinea (Coleoptera, Dytiscidae, Copelatinae).

Project description:Six new species of New Guinea Exocelina Broun, 1886 are described in this paper: E. iratoisp. n., E. likuisp. n., E. puisp. n., E. pulukensissp. n., E. tomhansisp. n., and E. wigodukensissp. n. Although different morphologically, together with Exocelina ascendens (Balke, 1998), E. bagus (Balke & Hendrich, 2001), and E. ransikiensis Shaverdo, Panjaitan & Balke, 2016, they are found to form a monophyletic clade and be closely related to representatives of the E. ekari-group, based on preliminary analysis of sequence data. An identification key to the species is provided, and important diagnostic characters are illustrated. The present data on the species' distribution show that most of them are local endemics.

| S-EPMC5523169 | biostudies-literature

Nine new species groups, 15 new species, and one new subspecies of New Guinea diving beetles of the genus Exocelina Broun, 1886 (Coleoptera, Dytiscidae, Copelatinae).

Project description:Nine new species groups of Exocelina Broun, 1886 from New Guinea are introduced with keys to their representatives. Four groups are monotypic and include three new species: the E. aipomek group, the E. koroba group: E. koroba sp. nov., the E. mekilensis group: E. mekilensis sp. nov., and the E. morobensis group: E. morobensis sp. nov. The remaining five species groups include 18 species with 12 new species and one new subspecies: the E. bacchusi group: E. akameku sp. nov., E. oiwa sp. nov., E. oksibilensis sp. nov., and E. bacchusi herzogensis ssp. nov.; the E. jaseminae group: E. aseki sp. nov., E. kailaki sp. nov., and E. pseudojaseminae sp. nov.; the E. larsoni group: E. warahulenensis sp. nov.; the E. takime group: E. mianminensis sp. nov.; and the E. warasera group: E. haia sp. nov., E. kobau sp. nov., E. pulchella sp. nov., and E. warasera sp. nov. Diagnoses of five already described species of these groups are provided, as well as comparatives notes on all species. Exocelina santimontis (Balke, 1998) syn. nov. is a junior synonym of E. aipomek (Balke, 1998). Data on the distribution of the species are given, showing that most of the species of these groups occur in the Papua New Guinea.

| S-EPMC6791902 | biostudies-other

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data