Selection of appropriate protein assay method for a paper microfluidics platform.

ABSTRACT:

Background

Paper-analytical devices (PADs) have gained popularity as a simple and low-cost alternative for determining a wide range of analytes including proteins. Even though several colorimetric PADs methods for protein estimation are reported in literature, they lack justification for the chosen method and parameters therein.

Aim

Major aim of this work was to thoroughly evaluate the most commonly used colorimetric protein assays and recommend the most appropriate method for PADs platform.

Method

We performed following six colorimetric protein assays on PADs: biuret, lowry, bicinchoninic acid, bradford, bromocresol green, and tetrabromophenol blue. We obtained assay signal by analyzing images of the PADs and then assessed analytical figures of merit.

Result

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SUBMITTER: Pokhrel P 

PROVIDER: S-EPMC7248653 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature