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Development of 16 microsatellite markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae).


ABSTRACT: The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.

SUBMITTER: Liu Z 

PROVIDER: S-EPMC3344197 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Development of 16 microsatellite markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae).

Liu Zhenwen Z   Zhao Qianru Q   Zhou Jing J   Peng Hua H   Yang Junbo J  

International journal of molecular sciences 20120323 4


The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expect  ...[more]

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