Dataset Information

LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells.

ABSTRACT:

Aim

Our study is aimed at investigating whether Lipopolysaccharide- (LPS-) treated podocytes could polarize naive CD4⁺ T cells into different subsets in vitro.

Materials and methods

Podocytes and mouse bone marrow-derived dendritic cells (BMDCs) were first cultured with 25??g/ml LPS for 6 hours, respectively. Then, naive CD4⁺ T cells were cocultured with the LPS-treated podocytes or BMDCs at a ratio of 1?:?1 or 1?:?1?:?1. After 48 hours, we collected the suspended cells and supernatant from all groups to measure T helper (Th)17 cells, regulatory T (Treg) cells, and cytokine concentration.

Results

We observed the expression of CD80 and major histocompatibility complex class II molecule (MHC II) in podocytes but did not found the upregulation of them after treating podocytes with LPS. LPS-treated podocytes could induce naive CD4⁺ T cells to Th17 cells and Treg cells with a higher ratio of Th17/Treg than BMDCs. Possible interaction between podocytes and BMDCs may exist in the induction process of Th17 cells and Treg cells.

Conclusion

Our study proved that CD80 and MHC II were constitutively expressed in podocytes but not upregulated by LPS. LPS-treated podocytes could polarize naive CD4⁺ T cells into Th17 and Treg cells and affect the Th17/Treg balance and may incline to cause a Th17 response.

SUBMITTER: Yuan DH

PROVIDER: S-EPMC7251438 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

LPS-Treated Podocytes Polarize Naive CD4<sup>+</sup> T Cells into Th17 and Treg Cells.

Yuan Da-Hai DH Jia Yang Y Hassan Omar Mohamud OM Xu Li-Yun LY Wu Xiao-Chuan XC

BioMed research international 20200517

<h4>Aim</h4>Our study is aimed at investigating whether Lipopolysaccharide- (LPS-) treated podocytes could polarize naive CD4<sup>+</sup> T cells into different subsets in vitro.<h4>Materials and methods</h4>Podocytes and mouse bone marrow-derived dendritic cells (BMDCs) were first cultured with 25 <i>μ</i>g/ml LPS for 6 hours, respectively. Then, naive CD4<sup>+</sup> T cells were cocultured with the LPS-treated podocytes or BMDCs at a ratio of 1 : 1 or 1 : 1 : 1. After 48 hours, we collected t ...[more]

PMID: 32509873

Similar Datasets

Project description:Background: Patients with predominantly antibody deficiency (PAD) suffer from severe and recurrent infections that require lifelong immunoglobulin replacement and prophylactic antibiotic treatment. Disease incidence is estimated to be 1:25,000 worldwide, and up to 68% of patients develop non-infectious complications (NIC) including autoimmunity, which are difficult to treat, causing high morbidity, and early mortality. Currently, the etiology of NIC is unknown, and there are no diagnostic and prognostic markers to identify patients at risk. Objectives: To identify immune cell markers that associate with NIC in PAD patients. Methods: We developed a standardized 11-color flow cytometry panel that was utilized for in-depth analysis of B and T cells in 62 adult PAD patients and 59 age-matched controls. Results: Nine males had mutations in Bruton's tyrosine kinase (BTK) and were defined as having X-linked agammaglobulinemia. The remaining 53 patients were not genetically defined and were clinically diagnosed with agammaglobulinemia (n = 1), common variable immunodeficiency (CVID) (n = 32), hypogammaglobulinemia (n = 13), IgG subclass deficiency (n = 1), and specific polysaccharide antibody deficiency (n = 6). Of the 53, 30 (57%) had one or more NICs, 24 patients had reduced B-cell numbers, and 17 had reduced T-cell numbers. Both PAD-NIC and PAD+NIC groups had significantly reduced Ig class-switched memory B cells and naive CD4 and CD8 T-cell numbers. Naive and IgM memory B cells, Treg, Th17, and Tfh17 cells were specifically reduced in the PAD+NIC group. CD21lo B cells and Tfh cells were increased in frequencies, but not in absolute numbers in PAD+NIC. Conclusion: The previously reported increased frequencies of CD21lo B cells and Tfh cells are the indirect result of reduced naive B-cell and T-cell numbers. Hence, correct interpretation of immunophenotyping of immunodeficiencies is critically dependent on absolute cell counts. Finally, the defects in naive B- and T-cell numbers suggest a mild combined immunodeficiency in PAD patients with NIC. Together with the reductions in Th17, Treg, and Tfh17 numbers, these key differences could be utilized as biomarkers to support definitive diagnosis and to predict for disease progression.

Dataset Information

LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells.

Aim

Materials and methods

Results

Conclusion

Publications

LPS-Treated Podocytes Polarize Naive CD4<sup>+</sup> T Cells into Th17 and Treg Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

LPS-Treated Podocytes Polarize Naive CD4+ T Cells into Th17 and Treg Cells.

Aim

Materials and methods

Results

Conclusion

Publications

LPS-Treated Podocytes Polarize Naive CD4<sup>+</sup> T Cells into Th17 and Treg Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

LPS-Treated Podocytes Polarize Naive CD4⁺ T Cells into Th17 and Treg Cells.