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A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue.

ABSTRACT: Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) ?g of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 ?g and 0.48 ± 0.43 ?g per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.

SUBMITTER: Wei S

PROVIDER: S-EPMC7252492 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue.

Wei Shan S Levy Brynn B Hoffman Nataly N Cujar Claudia C Rodney-Sandy Reunet R Wapner Ronald R D'Alton Mary M Williams Zev Z

BioTechniques 20200214 5

Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-m ...[more]

PMID: 32054310

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