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A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis.

ABSTRACT: A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200-450?ng/?l) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.

SUBMITTER: Harrison DJA

PROVIDER: S-EPMC7474859 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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A rapid and low-cost method for genomic DNA extraction from the cyanobacterium <i>Synechocystis</i>.

Harrison Dale J A DJA Thompson Elinor P EP

Biology methods & protocols 20200613 1

A two-step method is reported for preparation of genomic DNA from the model cyanobacterium <i>Synechocystis</i> that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200-450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest i ...[more]

PMID: 32913895

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