Dataset Information
Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering.
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ABSTRACT: Background
The development of a biological based small diameter vascular graft (d?MethodsHuman UAs were decellularized using 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) detergents. Then, vitrified with vitrification solution 55 (VS55) solution, remained for 6 months in liquid nitrogen and their extracellular matrix composition was compared to conventionally cryopreserved UAs. Additionally, total hydroxyproline, sulphated glycosaminoglycan and DNA content were quantified in all samples. Finally, the vitrified umbilical arteries implanted as common carotid artery interposition graft to a porcine animal model.Results
Decellularized and vitrified UAs characterized by proper preservation of extracellular matrix proteins and tissue architecture, whereas conventionally cryopreserved samples exhibited a disorganized structure. Total hydroxyproline content was preserved, although sulphated glycosaminoglycan and DNA contents presented significantly alterations in all samples. Implanted UAs successfully recellularized and remodeled as indicated by the histological analysis.Conclusion
Decellularized and vitrified UAs retained their structure function properties and can be possible used as an alternative source for readily accessible small diameter vascular grafts.
SUBMITTER: Mallis P
PROVIDER: S-EPMC7260347 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering.
Tissue engineering and regenerative medicine 20200313 3
<h4>Background</h4>The development of a biological based small diameter vascular graft (d < 6 mm), that can be properly stored over a long time period at - 196 °C, in order to directly be used to the patients, still remains a challenge. In this study the decellularized umbilical arteries (UAs) where vitrified, evaluated their composition and implanted to a porcine model, thus serving as vascular graft.<h4>Methods</h4>Human UAs were decellularized using 3-[(3-cholamidopropyl)dimethylammonio]-1-pr ...[more]