Comparative immunological studies of tumor-associated Lewis X, Lewis Y, and KH-1 antigens.

ABSTRACT: Tumor-associated carbohydrate antigens Lewis X (Le^x), Lewis Y (Le^y), and KH-1 are useful targets for cancer immunotherapy. In this regard, an insight into the structure-immunogenicity relationships of these antigens is important but this has not been systematically investigated yet. In the current study, Le^x, Le^y, and KH-1 antigens with a lactose unit at the reducing end as a spacer were synthesized and coupled with keyhole limpet hemocyanin (KLH) protein. Immunological evaluations of the resultant conjugates revealed that they all could elicit robust immune responses whilst the Le^y conjugate could provoke the highest titers of total and IgG antibodies. The binding assays of their antisera to each antigen and to cancer cells showed tha

SUBMITTER: Guo J 

PROVIDER: S-EPMC7261630 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature