Unknown

Dataset Information

0

Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2.


ABSTRACT: The COVID-19 pandemic provides an urgent example where a gap exists between availability of state-of-the-art diagnostics and current needs. As assay details and primer sequences become widely known, many laboratories could perform diagnostic tests using methods such as RT-PCR or isothermal RT-LAMP amplification. A key advantage of RT-LAMP based approaches compared to RT-PCR is that RT-LAMP is known to be robust in detecting targets from unprocessed samples. In addition, RT-LAMP assays are performed at a constant temperature enabling speed, simplicity, and point-of-use testing. Here, we provide the details of an RT-LAMP isothermal assay for the detection of SARS-CoV-2 virus with performance comparable to currently approved tests using RT-PCR. We characterize the assay by introducing swabs in virus spiked synthetic nasal fluids, moving the swab to viral transport medium (VTM), and using a volume of that VTM for performing the amplification without an RNA extraction kit. The assay has a Limit-of-Detection (LOD) of 50 RNA copies/?L in the VTM solution within 20 minutes, and LOD of 5000 RNA copies/?L in the nasal solution. Additionally, we show the utility of this assay for real-time point-of-use testing by demonstrating detection of SARS-CoV-2 virus in less than 40 minutes using an additively manufactured cartridge and a smartphone-based reader. Finally, we explore the speed and cost advantages by comparing the required resources and workflows with RT-PCR. This work could accelerate the development and availability of SARS-CoV-2 diagnostics by proving alternatives to conventional laboratory benchtop tests.

SUBMITTER: Ganguli A 

PROVIDER: S-EPMC7263486 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2.

Ganguli A A   Mostafa A A   Berger J J   Aydin M M   Sun F F   Valera E E   Cunningham B T BT   King W P WP   Bashir R R  

bioRxiv : the preprint server for biology 20200521


The COVID-19 pandemic provides an urgent example where a gap exists between availability of state-of-the-art diagnostics and current needs. As assay details and primer sequences become widely known, many laboratories could perform diagnostic tests using methods such as RT-PCR or isothermal RT-LAMP amplification. A key advantage of RT-LAMP based approaches compared to RT-PCR is that RT-LAMP is known to be robust in detecting targets from unprocessed samples. In addition, RT-LAMP assays are perfor  ...[more]

Similar Datasets

| S-EPMC7502724 | biostudies-literature
| S-EPMC9759211 | biostudies-literature
| S-EPMC8023013 | biostudies-literature
| S-EPMC8688429 | biostudies-literature
| S-EPMC8437165 | biostudies-literature
| S-EPMC7275676 | biostudies-literature
| S-EPMC7216271 | biostudies-literature
| S-EPMC7097549 | biostudies-literature
| S-EPMC7552048 | biostudies-literature
| S-EPMC8008787 | biostudies-literature