DeCoDe: degenerate codon design for complete protein-coding DNA libraries.

ABSTRACT:

Motivation

High-throughput protein screening is a critical technique for dissecting and designing protein function. Libraries for these assays can be created through a number of means, including targeted or random mutagenesis of a template protein sequence or direct DNA synthesis. However, mutagenic library construction methods often yield vastly more nonfunctional than functional variants and, despite advances in large-scale DNA synthesis, individual synthesis of each desired DNA template is often prohibitively expensive. Consequently, many protein-screening libraries rely on the use of degenerate codons (DCs), mixtures of DNA bases incorporated at specific positions during DNA synthesis, to generate highly diverse protein-variant pools from only a few low-cost synthesis reactions

SUBMITTER: Shimko TC 

PROVIDER: S-EPMC7267834 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature