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Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling.

ABSTRACT: OBJECTIVE:Microbial community profiling using 16S rRNA gene has provided invaluable insights into diverse microbial communities. Recently a few studies have attempted to use 16S rRNA gene microbiota profiling in combination with the conventional culture methods to explore bacterial communities. In this "culture-enriched microbiota profiling" approach, microbes in a sample are cultured on solid media, and the resulting colonies are combined and subjected to 16S rRNA gene microbiota profiling. Here we investigated the effect of cell densities as determined by varying levels of sample dilution on the culture-enriched microbiota profiles using De Man, Rogosa and Sharpe (MRS) agar medium as a model system. RESULTS:Cecal samples collected from 10 healthy chickens were serially diluted to 102 fold (M-LOW), 104 fold (M-MEDIUM), and 106 fold (M-HIGH), and the dilutions were plated on MRS agar. 16S rRNA gene profiling showed that the relative abundance of certain genera showed gradual increase (Pediococcus and Enterococcus) or decrease (Lactobacillus and Turicibacter) with higher dilutions, though it was significant only for Pediococcus (p?

SUBMITTER: Adhikari B

PROVIDER: S-EPMC7268277 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling.

Adhikari Bishnu B Kwon Young Min YM

BMC research notes 20200603 1

<h4>Objective</h4>Microbial community profiling using 16S rRNA gene has provided invaluable insights into diverse microbial communities. Recently a few studies have attempted to use 16S rRNA gene microbiota profiling in combination with the conventional culture methods to explore bacterial communities. In this "culture-enriched microbiota profiling" approach, microbes in a sample are cultured on solid media, and the resulting colonies are combined and subjected to 16S rRNA gene microbiota profil ...[more]

PMID: 32493423

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Dataset Information

Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling.

Publications

Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling.

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