Anti-inflammatory Withanolides from Physalis minima.

ABSTRACT: Five new withanolides (1-5) along with five known ones (6-10) were isolated from the whole plants of Physalis minima Linn. The chemical structures of the new compounds were identified as (20S,22R) 15a-acetoxy-5?,6?-epoxy-4?,14a,28-trihydroxy-3?-methoxy-1-oxowitha-16,24-dienolide (1), (20S,22R) 15a-acetoxy-5?,6?-epoxy-3?,4?,14?,17?,20?-pentahydroxy-1-oxowitha-24-enolide (2), (20R,22R) 15?-acetoxy-4?,5?,6?,14?,20?-pentahydroxy-1-oxowitha-2,24-dienolide (3), (20R,22R) 15?-acetoxy-5?,6?,14?,20?-tetrahydroxy-1-oxowitha-2,24-dienolide (4), and (20S,22R) 5?,6?,14?-trihydroxy-1,15-dioxowitha-2,16,24-trienolide (5) on the basis of integration combining IR, UV, HR-ESI-MS, 1D-NMR, and 2D-NMR analyses. Biologically, compounds (1-10) were subjected to evaluate their anti-inflammatory activities via inhibiting nitric oxide production in lipopolysaccharide-stimulated murine RAW 264.7 cells in vitro. The activity screening indicated that all of the compounds showed a moderate inhibitory effect against nitric oxide production with IC₅₀ values of 23.53-66.28 ?M.

SUBMITTER: Wu J 

PROVIDER: S-EPMC7271383 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature