Ontology highlight
ABSTRACT:
SUBMITTER: Bai Y
PROVIDER: S-EPMC7279287 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Bai Yanfen Y Mansell Thomas J TJ
International journal of molecular sciences 20200520 10
The short-chain fatty acid butyrate plays critical roles in human gut health, affecting immunomodulation, cell differentiation, and apoptosis, while also serving as the preferred carbon source for colon cells. In this work, we have engineered a model probiotic organism, <i>Escherichia coli</i> Nissle 1917 (EcN, serotype O6:K5:H1), to produce butyrate from genomic loci up to approximately 1 g/L (11 mM). Then, for real-time monitoring of butyrate production in cultures, we developed a high-through ...[more]