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Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification.


ABSTRACT: The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, ?137-141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the ?137-141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase-75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the ?137-141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF ?137-141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.

SUBMITTER: Przybylski R 

PROVIDER: S-EPMC7281006 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification.

Przybylski Rémi R   Bazinet Laurent L   Firdaous Loubna L   Kouach Mostafa M   Goossens Jean-François JF   Dhulster Pascal P   Nedjar-Arroume Naïma N  

Membranes 20200503 5


The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137-141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial  ...[more]

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