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Mobilization and Cellular Distribution of Phosphate in the Diatom Phaeodactylum tricornutum.

ABSTRACT: Unicellular organisms that live in marine environments must cope with considerable fluctuations in the availability of inorganic phosphate (Pi). Here, we investigated the extracellular Pi concentration-dependent expression, as well as the intracellular or extracellular localization, of phosphatases and phosphate transporters of the diatom Phaeodactylum tricornutum. We identified Pi-regulated plasma membrane-localized, ER-localized, and secreted phosphatases, in addition to plasma membrane-localized, vacuolar membrane-localized, and plastid-surrounding membrane-localized phosphate transporters that were also regulated in a Pi concentration-dependent manner. These studies not only add further knowledge to already existing transcriptomic data, but also highlight the capacity of the diatom to distribute Pi intracellularly and to mobilize Pi from extracellular and intracellular resources.

SUBMITTER: Dell'Aquila G

PROVIDER: S-EPMC7283521 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Mobilization and Cellular Distribution of Phosphate in the Diatom Phaeodactylum tricornutum.

Dell'Aquila Gianluca G Zauner Stefan S Heimerl Thomas T Kahnt Jörg J Samel-Gondesen Vera V Runge Simon S Hempel Franziska F Maier Uwe G UG

Frontiers in plant science 20200603

Unicellular organisms that live in marine environments must cope with considerable fluctuations in the availability of inorganic phosphate (Pi). Here, we investigated the extracellular Pi concentration-dependent expression, as well as the intracellular or extracellular localization, of phosphatases and phosphate transporters of the diatom Phaeodactylum tricornutum. We identified Pi-regulated plasma membrane-localized, ER-localized, and secreted phosphatase ...[more]

PMID: 32582227

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Mobilization and cellular distribution of phosphate in the diatom Phaeodactylum tricornutum

Project description:Unicellular organisms living in a marine environment sometimes have to cope with massive fluctuations in inorganic phosphate (Pi) availability. We studied phosphatases and phosphate transporters of the diatom Phaeodactylum tricornutum with respect to the extracellular Pi-concentration depending expression as well as their extra- or intracellular localization. These studies, which add a further level of knowledge to already existing transcriptomic data, highlight the capacity of the diatom to distribute Pi intracellularly and to mobilize Pi from extra- and intracellular resources

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Localization and Evolution of Putative Triose Phosphate Translocators in the Diatom Phaeodactylum tricornutum.

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Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum.

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Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum.

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