Ontology highlight
ABSTRACT:
SUBMITTER: Jimenez-Sanchez C
PROVIDER: S-EPMC7286097 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Jimenez-Sanchez Celia C Wilson Nina N McMaster Nicole N Gantulga Dash D Freedman Benjamin G BG Senger Ryan R Schmale David G DG
Toxicon: X 20200123
New strategies are needed to mitigate the mycotoxin deoxynivalenol (DON) in feed and food products. Microbial DNA fragments were generated from a library of DON-tolerant microorganisms. These fragments were screened in DON-sensitive yeast strains for their ability to modify or transport DON. Fragments were cloned into a PCR8/TOPO vector, and recombined into the yeast vector, pYES-DEST52. Resulting yeast transformants were screened in the presence of 100 ppm DON. Transformants that were able to g ...[more]