Dataset Information

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

ABSTRACT: Viral canine diarrhea has high morbidity and mortality and is prevalent worldwide, resulting in severe economic and spiritual losses to pet owners. However, diarrhea pathogens have similar clinical symptoms and are difficult to diagnose clinically. Thus, fast and accurate diagnostic methods are of great significance for prevention and accurate treatment. In this study, we developed a one-step multiplex TaqMan probe-based real-time PCR for the differential diagnosis of four viruses causing canine diarrhea including, CPV (Canine Parvovirus), CCoV (Canine Coronavirus), CAstV (Canine Astrovirus), and CaKoV (Canine Kobuviruses). The limit of detection was up to 102 copies/?L and performed well with high sensitivity and specificity. This assay was optimized and used to identify possible antagonistic relationships between viruses. From this, artificial pre-experiments were performed for mixed infections, and a total of 82 canine diarrhea field samples were collected from different animal hospitals in Zhejiang, China to assess the method. The virus prevalence was significantly higher than what previously reported based on RT-PCR (Reverse Transcription-Polymerase Chain Reaction). Taken together, these results suggest that the method can be used as a preferred tool for monitoring laboratory epidemics, timely prevention, and effective monitoring of disease progression.

SUBMITTER: Wang R

PROVIDER: S-EPMC7286240 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

Wang Ruyi R Zhang Wenyan W Ye Rui R Pan Zhongzhou Z Li Gairu G Su Shuo S

Molecular and cellular probes 20200610

Viral canine diarrhea has high morbidity and mortality and is prevalent worldwide, resulting in severe economic and spiritual losses to pet owners. However, diarrhea pathogens have similar clinical symptoms and are difficult to diagnose clinically. Thus, fast and accurate diagnostic methods are of great significance for prevention and accurate treatment. In this study, we developed a one-step multiplex TaqMan probe-based real-time PCR for the differential diagnosis of four viruses causing canine ...[more]

PMID: 32534013

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One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

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One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

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