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Salt-Mediated Nanopore Detection of ADAM-17.

ABSTRACT: ADAM-17 (a disintegrin and metalloproteinase 17) plays an important role in various physiological and pathophysiological processes. Overexpression/underexpression of ADAM-17 could lead to various diseases. In this work, by taking advantage of ionic strength and salt gradient, and monitoring the cleavage of a substrate peptide by ADAM-17 in a nanopore, we developed a label-free sensor for the rapid detection of ADAM-17. The sensor was highly sensitive and selective: picomolar concentrations of ADAM-17 could be detected within minutes, while structure similar proteases such as ADAM-9 and MMP-9 did not interfere with its detection. Our developed nanopore sensing strategy should find useful applications in the development of nanopore sensors for other proteases of biological, pharmaceutical, and medical importance.

SUBMITTER: Chen X

PROVIDER: S-EPMC7288862 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Salt-Mediated Nanopore Detection of ADAM-17.

Chen Xiaohan X Zhang Youwen Y Roozbahani Golbarg Mohammadi GM Guan Xiyun X

ACS applied bio materials 20181224 1

ADAM-17 (a disintegrin and metalloproteinase 17) plays an important role in various physiological and pathophysiological processes. Overexpression/underexpression of ADAM-17 could lead to various diseases. In this work, by taking advantage of ionic strength and salt gradient, and monitoring the cleavage of a substrate peptide by ADAM-17 in a nanopore, we developed a label-free sensor for the rapid detection of ADAM-17. The sensor was highly sensitive and selective: picomolar concentrations of AD ...[more]

PMID: 32529174

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