Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC7289059 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Chen Jin J Brunner Andreas-David AD Cogan J Zachery JZ Nuñez James K JK Fields Alexander P AP Adamson Britt B Itzhak Daniel N DN Li Jason Y JY Mann Matthias M Leonetti Manuel D MD Weissman Jonathan S JS
Science (New York, N.Y.) 20200301 6482
Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based screening strategy to identify hundreds of noncanonical CDSs that are essential for cellular growth and whose disruption elicits specific, robust transcriptomic and phenotypic changes in human cells. Functional characterization of the encoded microproteins reveals distinct cellular localizations, specific protein bindin ...[more]