Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.
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ABSTRACT:
SUBMITTER: Mishra K
PROVIDER: S-EPMC7292636 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.
Mishra Kanuj K Stankevych Mariia M Fuenzalida-Werner Juan Pablo JP Grassmann Simon S Gujrati Vipul V Huang Yuanhui Y Klemm Uwe U Buchholz Veit R VR Ntziachristos Vasilis V Stiel Andre C AC
Science advances 20200612 24
We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences. ...[more]
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