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Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

ABSTRACT: A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at single-cell level within a heterogeneous population. We present Met-Flow, a flow cytometry-based method capturing the metabolic state of immune cells by targeting key proteins and rate-limiting enzymes across multiple pathways. We demonstrate the ability to simultaneously measure divergent metabolic profiles and dynamic remodeling in human peripheral blood mononuclear cells. Using Met-Flow, we discovered that glucose restriction and metabolic remodeling drive the expansion of an inflammatory central memory T cell subset. This method captures the complex metabolic state of any cell as it relates to phenotype and function, leading to a greater understanding of the role of metabolic heterogeneity in immune responses.

SUBMITTER: Ahl PJ

PROVIDER: S-EPMC7292829 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

Ahl Patricia J PJ Hopkins Richard A RA Xiang Wen Wei WW Au Bijin B Kaliaperumal Nivashini N Fairhurst Anna-Marie AM Connolly John E JE

Communications biology 20200612 1

A complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond to changing environments by metabolic reprogramming is crucial to effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at single-cell level within a heterogeneous population. We present Met-Flow, a flow cytometry-based method capturing the metabolic state of immune cells by targeting key pro ...[more]

PMID: 32533056

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Dataset Information

Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

Publications

Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

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