Ontology highlight
ABSTRACT:
SUBMITTER: Kneuttinger AC
PROVIDER: S-EPMC7293202 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Kneuttinger Andrea C AC Straub Kristina K Bittner Philipp P Simeth Nadja A NA Bruckmann Astrid A Busch Florian F Rajendran Chitra C Hupfeld Enrico E Wysocki Vicki H VH Horinek Dominik D König Burkhard B Merkl Rainer R Sterner Reinhard R
Cell chemical biology 20190905 11
Imidazole glycerol phosphate synthase (ImGPS) is an allosteric bienzyme complex in which substrate binding to the synthase subunit HisF stimulates the glutaminase subunit HisH. To control this stimulation with light, we have incorporated the photo-responsive unnatural amino acids phenylalanine-4'-azobenzene (AzoF), o-nitropiperonyl-O-tyrosine (NPY), and methyl-o-nitropiperonyllysine (mNPK) at strategic positions of HisF. The light-mediated isomerization of AzoF at position 55 (fS55AzoF<sup>E</su ...[more]