Ontology highlight
ABSTRACT:
SUBMITTER: Riggan L
PROVIDER: S-EPMC7294876 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Riggan Luke L Hildreth Andrew D AD Rolot Marion M Wong Yung-Yu YY Satyadi William W Sun Ryan R Huerta Christopher C O'Sullivan Timothy E TE
Cell reports 20200501 7
CRISPR genome engineering has become a powerful tool to functionally investigate the complex mechanisms of immune system regulation. While decades of work have aimed to genetically reprogram innate immunity, the utility of current approaches is restricted by poor knockout efficiencies or limited specificity for mature cell lineages in vivo. Here, we describe an optimized strategy for non-viral CRISPR-Cas9 ribonucleoprotein (cRNP) genomic editing of mature primary mouse innate lymphocyte cells (I ...[more]