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Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.

ABSTRACT: The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARSCoV-2-specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1) and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies.

SUBMITTER: Schmidt F

PROVIDER: S-EPMC7302213 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.

Schmidt Fabian F Weisblum Yiska Y Muecksch Frauke F Hoffmann Hans-Heinrich HH Michailidis Eleftherios E Lorenzi Julio C C JCC Mendoza Pilar P Rutkowska Magdalena M Bednarski Eva E Gaebler Christian C Agudelo Marianna M Cho Alice A Wang Zijun Z Gazumyan Anna A Cipolla Melissa M Caskey Marina M Robbiani Davide F DF Nussenzweig Michel C MC Rice Charles M CM Hatziioannou Theodora T Bieniasz Paul D PD

bioRxiv : the preprint server for biology 20200609

The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARSCoV-2-specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1) and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate viru ...[more]

PMID: 32577658

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Dataset Information

Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.

Publications

Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.

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