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HFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.

ABSTRACT: Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5?mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.

SUBMITTER: Hildebrand S

PROVIDER: S-EPMC7305111 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.

Hildebrand Sven S Schueth Anna A Wangenheim Klaus von KV Mattheyer Christian C Pampaloni Francesco F Bratzke Hansjürgen H Roebroeck Alard F AF Galuske Ralf A W RAW

Scientific reports 20200619 1

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) ...[more]

PMID: 32561795

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