Optical tissue clearing and machine learning can precisely characterize extravasation and blood vessel architecture in brain tumors.

ABSTRACT: Precise methods for quantifying drug accumulation in brain tissue are currently very limited, challenging the development of new therapeutics for brain disorders. Transcardial perfusion is instrumental for removing the intravascular fraction of an injected compound, thereby allowing for ex vivo assessment of extravasation into the brain. However, pathological remodeling of tissue microenvironment can affect the efficiency of transcardial perfusion, which has been largely overlooked. We show that, in contrast to healthy vasculature, transcardial perfusion cannot remove an injected compound from the tumor vasculature to a sufficient extent leading to considerable overestimation of compound extravasation. We demonstrate that 3D deep imaging of optically cleared tumor samples overcomes this li

SUBMITTER: Kostrikov S 

PROVIDER: S-EPMC8249617 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature