Ontology highlight
ABSTRACT:
SUBMITTER: Wannier TM
PROVIDER: S-EPMC7306799 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Wannier Timothy M TM Nyerges Akos A Kuchwara Helene M HM Czikkely Márton M Balogh Dávid D Filsinger Gabriel T GT Borders Nathaniel C NC Gregg Christopher J CJ Lajoie Marc J MJ Rios Xavier X Pál Csaba C Church George M GM Church George M GM
Proceedings of the National Academy of Sciences of the United States of America 20200528 24
Exploiting bacteriophage-derived homologous recombination processes has enabled precise, multiplex editing of microbial genomes and the construction of billions of customized genetic variants in a single day. The techniques that enable this, multiplex automated genome engineering (MAGE) and directed evolution with random genomic mutations (DIvERGE), are however, currently limited to a handful of microorganisms for which single-stranded DNA-annealing proteins (SSAPs) that promote efficient recomb ...[more]