Ontology highlight
ABSTRACT:
SUBMITTER: Zosel F
PROVIDER: S-EPMC7306994 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Zosel Franziska F Soranno Andrea A Buholzer Karin J KJ Nettels Daniel D Schuler Benjamin B
Proceedings of the National Academy of Sciences of the United States of America 20200602 24
Intrinsically disordered proteins (IDPs) abound in cellular regulation. Their interactions are often transitory and highly sensitive to salt concentration and posttranslational modifications. However, little is known about the effect of macromolecular crowding on the interactions of IDPs with their cellular targets. Here, we investigate the influence of crowding on the interaction between two IDPs that fold upon binding, with polyethylene glycol as a crowding agent. Single-molecule spectroscopy ...[more]