Dataset Information

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China.

ABSTRACT: Background and Aim:As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods:A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results:The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion:This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.

SUBMITTER: Mengfan Q

PROVIDER: S-EPMC7311875 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Molecular detection and genetic variability of <i>Ehrlichia canis</i> in pet dogs in Xinjiang, China.

Mengfan Qiao Q Lixia Wang W Ying Lei L Yan Ren R Kuojun Cai C Jinsheng Zhang Z Zaichao Zhang Z Weiwei Yu Y Yelong Peng P Xuepeng Cai C Chongyang Li L Jun Qiao Q Qingling Meng M

Veterinary world 20200518 5

<h4>Background and aim</h4>As a tick-borne zoonotic pathogen, <i>Ehrlichia canis</i> has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of <i>E. canis</i> in pet dogs in Xinjiang, China.<h4>Materials and methods</h4>A total of 297 blood samples of pet dogs and 709 skin ticks (<i>Rhipicephalus sanguineus sensu lato</i>) were subjected to molecular detection using PCR for <i>E. canis</i> 16S <i>rRNA</i> gene, and then, p ...[more]

PMID: 32636588

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Project description:BackgroundPet dogs are important companion animals that share the environment within households, and play an important role in local community life. In addition, pet dogs also are reservoirs of zoonotic agents, including Rickettsia spp., thus increasing the risk of rickettsial infections in humans. It's meaningful to investigate the epidemiology of rickettsial agents in pet dogs, and make contribute to the surveillance of rickettsioses in human in China.ResultsIn this study, a total of 496 pet dogs' blood samples and 343 ticks infested in pet dogs were collected, and the presence and prevalence of Rickettsia were determined by amplifying the partial gltA and 17-kDa genes, with an overall positive rate of 8.1 % in blood samples and 14.0 % in tick samples. In addition, the rrs, gltA, groEL, and ompA genes of rickettsial were also recovered to determine the species of Rickettsia detected furtherly. Sequencing blast and phylogenetic analyses revealed the presence of three human pathogenic Rickettsia species (Rickettsia raoultii, Candidatus Rickettsia tarasevichiae and Rickettsia felis) in samples associated with pet dogs. Moreover, all the sequences of Rickettsia that we obtained presented close relationship with others available in GenBank, and Rickettsia raoultii was the most predominant Rickettsia species infected in pet dogs' blood samples or in tick samples.ConclusionsThis study provides the molecular epidemiology data about the Rickettsia spp. infection associated with pet dogs in urban areas of Harbin city. Three rickettisae species pathogenic to humans were identified from pet dogs' blood and the infested ticks in urban areas of Harbin city. Considering the intimate relationship between human and pets, these results indicate the potential transmission risk of human rickettisal infections from pet dogs through ectoparasites, and also highlighting that more attention should be paid to rickettsial infection in pet dogs and the infested ticks from the "One health" perspective.

Dataset Information

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China.

Publications

Molecular detection and genetic variability of <i>Ehrlichia canis</i> in pet dogs in Xinjiang, China.

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