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Attempting to synthesize lasso peptides using high pressure.

ABSTRACT: Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14 000 kbar.

SUBMITTER: Waliczek M

PROVIDER: S-EPMC7314030 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Attempting to synthesize lasso peptides using high pressure.

Waliczek Mateusz M Wierzbicka Magdalena M Arkuszewski Maciej M Kijewska Monika M Jaremko Łukasz Ł Rajagopal Priyadharshni P Szczepski Kacper K Sroczyńska Amanda A Jaremko Mariusz M Stefanowicz Piotr P

PloS one 20200624 6

Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing t ...[more]

PMID: 32579565

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