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Structure Validation of G-Rich RNAs in Noncoding Regions of the Human Genome.

ABSTRACT: We present the rapid biophysical characterization of six previously reported putative G-quadruplex-forming RNAs from the 5'-untranslated region (5'-UTR) of silvestrol-sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence-[AGG]₂ [CGG]₂ C-folds into a single well-defined G-quadruplex structure. Sequences with longer poly-G strands form unspecific aggregates, whereas CGG-repeat-containing sequences exhibit a temperature-dependent equilibrium between a hairpin and a G-quadruplex structure. The applied experimental strategy is fast and provides robust readout for G-quadruplex-forming capacities of RNA oligomers.

SUBMITTER: Binas O

PROVIDER: S-EPMC7318348 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Structure Validation of G-Rich RNAs in Noncoding Regions of the Human Genome.

Binas Oliver O Bessi Irene I Schwalbe Harald H

Chembiochem : a European journal of chemical biology 20200226 11

We present the rapid biophysical characterization of six previously reported putative G-quadruplex-forming RNAs from the 5'-untranslated region (5'-UTR) of silvestrol-sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence-[AGG]<sub>2</sub> [CGG]<sub>2</sub> C-folds into a single well-defined G-quadruplex structure. Sequences with longer poly-G strands form unspecific aggregates, whereas CGG-repeat-containi ...[more]

PMID: 31943589

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