Unknown

Dataset Information

0

Nosocomial outbreak of the Middle East Respiratory Syndrome coronavirus: A phylogenetic, epidemiological, clinical and infection control analysis.


ABSTRACT: BACKGROUND:Middle East Respiratory Syndrome coronavirus (MERS-CoV) continues to cause intermittent community and nosocomial outbreaks. Obtaining data on specific source(s) and transmission dynamics of MERS-CoV during nosocomial outbreaks has been challenging. We performed a clinical, epidemiological and phylogenetic investigation of an outbreak of MERS-CoV at a University Hospital in Riyadh, Kingdom of Saudi Arabia. METHODS:Clinical, epidemiological and infection control data were obtained from patients and Healthcare workers (HCWs). Full genome sequencing was conducted on nucleic acid extracted directly from MERS-CoV PCR-confirmed clinical samples and phylogenetic analysis performed. Phylogenetic analysis combined with published MERS-CoV genomes was performed. HCWs compliance with infection control practices was also assessed. RESULTS:Of 235 persons investigated, there were 23 laboratory confirmed MERS cases, 10 were inpatients and 13 HCWs. Eight of 10 MERS inpatients died (80% mortality). There were no deaths among HCWs. The primary index case assumed from epidemiological investigation was not substantiated phylogenetically. 17/18 MERS cases were linked both phylogenetically and epidemiologically. One asymptomatic HCW yielded a MERS-CoV genome not directly linked to any other case in the investigation. Five HCWs with mild symptoms yielded >75% full MERS-CoV genome sequences. HCW compliance with use of gowns was 62.1%, gloves 69.7%, and masks 57.6%. CONCLUSIONS:Several factors and sources, including a HCW MERS-CoV 'carrier phenomenon', occur during nosocomial MERS-CoV outbreaks. Phylogenetic analyses of MERS-CoV linked to clinical and epidemiological information is essential for outbreak investigation. The specific role of apparently healthy HCWs in causing nosocomial outbreaks requires further definition.

SUBMITTER: Barry M 

PROVIDER: S-EPMC7319941 | biostudies-literature | 2020 Sep - Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Nosocomial outbreak of the Middle East Respiratory Syndrome coronavirus: A phylogenetic, epidemiological, clinical and infection control analysis.

Barry Mazin M   Phan My Vt MV   Akkielah Layan L   Al-Majed Fahad F   Alhetheel Abdulkarim A   Somily Ali A   Alsubaie Sarah Suliman SS   McNabb Scott Jn SJ   Cotten Matthew M   Zumla Alimuddin A   Memish Ziad A ZA  

Travel medicine and infectious disease 20200627


<h4>Background</h4>Middle East Respiratory Syndrome coronavirus (MERS-CoV) continues to cause intermittent community and nosocomial outbreaks. Obtaining data on specific source(s) and transmission dynamics of MERS-CoV during nosocomial outbreaks has been challenging. We performed a clinical, epidemiological and phylogenetic investigation of an outbreak of MERS-CoV at a University Hospital in Riyadh, Kingdom of Saudi Arabia.<h4>Methods</h4>Clinical, epidemiological and infection control data were  ...[more]

Similar Datasets

| S-EPMC4029105 | biostudies-literature
| S-EPMC4690341 | biostudies-literature
| S-EPMC4558490 | biostudies-literature
| S-EPMC7106553 | biostudies-literature
| S-EPMC7087023 | biostudies-literature
| S-EPMC4734539 | biostudies-literature
2014-06-10 | E-GEOD-55023 | biostudies-arrayexpress
| S-EPMC6986839 | biostudies-literature
| PRJNA517346 | ENA
| PRJNA681679 | ENA